Fbxo15 knockout and CAG-EGFP transgenic double mutant mice. Fbxo15, F-box protein 15 is a novel target of undifferentiated-cell-specific transcripton factor Oct3/4 playing essential roles in self-renewal of ES cells and mouse early development. Fbxo15 is predominantly expressed in mouse undifferentiated ES cells. Inactivation of Oct3/4 in ES cells lead to rapid disappearance of Fbxo15 expression. 奈良先端科学技術大学院大学・山中伸弥先生（2004）。ICR-Tg(CAG-EGFP)1YamとB6;129S4- Fbxo15<tm1Yam>マウスとの交配により作出。混合背景。 Kyoto Univ. Developed by Shinya Yamanaka at Nara Institute of Science and Technology in 2004. Mixed background. KO: Developed by Dr. Shinya Yamanaka at Nara Institute of Science and Technology in 2003. A beta-geo cassette was inserted into the exon 3 through 7. The mutant mice were backcross to C57BL/6J for several times. Tg: The EGFP under the CAG promoter was injected into the pronuclei of fertilized eggs. ICR background. STOCK Tg(CAG-EGFP)1Yam Fbxo15<tm1Yam> Fbxo15遺伝子のノックアウトとCAG-EGFPトランスジェニックのダブルミュータントマウス系統。 Tg: Carrier x Noncarrier; KO: Homozygote x Homozygote [or Crossing to C57BL/6JJcl] Tg: Carrier x Noncarrier; KO: Homozygote x Homozygote [or Crossing to C57BL/6JJcl] true RF8 [129S4/SvJae] 国立大学法人京都大学 Shinya YAMANAKA STOCK Tg(CAG-EGFP)1Yam Fbxo15tm1Yam Developmental Biology Research Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: Japanese (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/01969_form_5.docx">Word</A>) / English(<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/01969_form_C.docx">Word</A>)</li><li>CAGGS MTA (<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/CAGGS_MTA.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol> C（3〜6か月） C (3-6 months) RBRC01969 CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), green flourescent protein (jellyfish)、E. coli lacZ, E. coli neo, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), SV40 polyA, mouse Fbxo15 genomic DNA 山中　伸弥